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Clinical Chemistry 31: 1127-1130, 1985;
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Clinical Chemistry, Vol 31, 1127-1130, Copyright © 1985 by American Association for Clinical Chemistry

Direct solid-phase radioimmunoassay for screening 17 alpha- hydroxyprogesterone in whole-blood samples from newborns

LF Hofman, JE Klaniecki and EK Smith

We describe a direct, solid-phase RIA for 17 alpha-hydroxyprogesterone (17-OH-P) that we are using to screen neonates for congenital adrenal hyperplasia. Phosphate buffer containing danazol and anti-17-OH-P is placed in tubes coated with antibody to IgG. The tubes also contain standards, controls, or blood samples on filter paper discs 3 mm in diameter. 125I-labeled 17-OH-P is added to each tube. The mixture is vortex-mixed and incubated overnight. The fluid and disc are removed, the radioactivity remaining in the tubes is counted, and the amount of 17-OH-P per disc is calculated by using a log-logit transformation of the standard curve. Results compare favorably with those by two extraction assays. Inter- and intra-assay CVs were less than 11% and less than 9%, respectively. Sensitivity was 2 pg per assay tube. There is no significant cross reactivity with structurally related steroids at their physiological concentrations. Analytical recovery of added 17- OH-P averaged 104%. 17-OH-P in whole blood spotted on filter paper is stable for at least six months.


The following articles in journals at HighWire Press have cited this article:


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 Clin. Chem.Home page
D. Torok, A. Muhl, F. Votava, G. Heinze, J. Solyom, J. Crone, S. Stockler-Ipsiroglu, and F. Waldhauser
Stability of 17{alpha}-Hydroxyprogesterone in Dried Blood Spots after Autoclaving and Prolonged Storage
Clin. Chem., February 1, 2002; 48(2): 370 - 372.
[Full Text] [PDF]

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C. J. Howe and D. J. Handelsman
Use of filter paper for sample collection and transport in steroid pharmacology
Clin. Chem., August 1, 1997; 43(8): 1408 - 1415.
[Abstract] [Full Text] [PDF]


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Copyright © 1985 by the American Association for Clinical Chemistry.