Clinical Chemistry Link to Randox Laboratories Web Site
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 31: 1163-1167, 1985;
This Article
Right arrow Full Text (PDF)
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lockwood, W. H.
Right arrow Articles by Curnow, D. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lockwood, W. H.
Right arrow Articles by Curnow, D. H.

Clinical Chemistry, Vol 31, 1163-1167, Copyright © 1985 by American Association for Clinical Chemistry

Rapid procedure for fecal porphyrin assay

WH Lockwood, V Poulos, E Rossi and DH Curnow

Hydrochloric acid extraction of feces in the presence of ether yields an extract suitable for spectrophotometric estimation of total porphyrin and for further separation by "high-performance" liquid chromatography (HPLC) or thin-layer chromatography. A total porphyrin reference interval of less than 200 nmol/g dry weight of feces was established from data on 106 normal subjects on an unrestricted diet. Total fecal porphyrin values in 11 porphyria cutanea tarda patients were considerably higher than given by the widely used Rimington method (respective means, 652 and 239 nmol/g dry weight). Our HPLC method for separation of porphyrin methyl esters on a silica column, with quantification by fluorescence, is described. HPLC separations performed on 23 porphyria cutanea tarda patients gave the following mean proportions of total fecal porphyrins: dicarboxylics 21%, coproporphyrin 9%, isocoproporphyrins 28%, pentacarboxylporphyrin 9%, hexacarboxylporphyrin 11%, heptacarboxylporphyrin 18%, and uroporphyrin 4%.


The following articles in journals at HighWire Press have cited this article:


Home page
 Arch DermatolHome page
C. Herrero, J. To-Figueras, C. Badenas, M. Mendez, P. Serrano, R. Enriquez-Salamanca, and M. Lecha
Clinical, Biochemical, and Genetic Study of 11 Patients With Erythropoietic Protoporphyria Including One With Homozygous Disease
Arch Dermatol, September 1, 2007; 143(9): 1125 - 1129.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
D.-L. Lin, L.-F. He, and Y.-Q. Li
Rapid and Simultaneous Determination of Coproporphyrin and Protoporphyrin in Feces by Derivative Matrix Isopotential Synchronous Fluorescence Spectrometry
Clin. Chem., October 1, 2004; 50(10): 1797 - 1803.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Pathol.Home page
A C Deacon and G H Elder
ACP Best Practice No 165: Front line tests for the investigation of suspected porphyria
J. Clin. Pathol., July 1, 2001; 54(7): 500 - 507.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
E. Rossi
Increased Fecal Porphyrins in Acute Intermittent Porphyria
Clin. Chem., February 1, 1999; 45(2): 281 - 300.
[Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1985 by the American Association for Clinical Chemistry.