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Clinical Chemistry 31: 1182-1184, 1985;
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Clinical Chemistry, Vol 31, 1182-1184, Copyright © 1985 by American Association for Clinical Chemistry

Rate-nephelometric determination of fibronectin in plasma

WC van Helden, A Kok-Verspuy, GA Harff and GJ van Kamp

We describe a kinetic immunonephelometric method for the determination of fibronectin in human plasma, used with the Beckman ICS rate nephelometer. The method is rapid and cost-effective. Two commercially available controls stated by the manufacturer to contain 200 and 295 mg/L were found to contain 198 and 290 mg/L, respectively. Mean analytical recovery was 104%. Within-run precision (CV) for normal samples was 3.8%, between-day precision 5.1%. For samples containing subnormal concentrations of fibronectin, these figures were 3.8% and 6.7%, respectively. Results by the method described here agreed and correlated well with those by a commercially available turbidimetric assay. With appropriately diluted samples, the range of measurement is 40 to 1000 mg/L. Normal values for women and men were 286 (SD 84) and 340 (SD 55) mg/L, respectively, in good agreement with values published by others.


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Mol. Cell. ProteomicsHome page
L. Anderson and C. L. Hunter
Quantitative Mass Spectrometric Multiple Reaction Monitoring Assays for Major Plasma Proteins
Mol. Cell. Proteomics, April 1, 2006; 5(4): 573 - 588.
[Abstract] [Full Text] [PDF]




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Copyright © 1985 by the American Association for Clinical Chemistry.