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Clinical Chemistry 31: 1203-1206, 1985;
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Clinical Chemistry, Vol 31, 1203-1206, Copyright © 1985 by American Association for Clinical Chemistry

Use of biotinylated probes for detecting sickle cell anemia

GJ Garbutt, JT Wilson, GS Schuster, JJ Leary and DC Ward

Earlier, we reported that the 5' end of the normal beta-globin gene (beta) resides on a 1.14-kilobase DNA fragment, whereas the 5' end of the sickle cell gene (beta s) resides on a 1.34-kilobase fragment. In that blot hybridization analysis, we used genomic DNA digested with restricted endonuclease Mst II, and radioactive probes with short half- life. We demonstrate here that, if a biotinylated probe is used instead in a slightly modified procedure, sickle cell anemia can be quickly and directly detected if there is as much as 5 micrograms of total genomic DNA in the sample. This procedure obviates the special precautions necessary when radioactive materials are used.




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Copyright © 1985 by the American Association for Clinical Chemistry.