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Clinical Chemistry 31: 1310-1313, 1985;
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Clinical Chemistry, Vol 31, 1310-1313, Copyright © 1985 by American Association for Clinical Chemistry

Spectrophotometric investigation of sensitive complexing agents for the determination of zinc in serum

R Homsher and B Zak

Of two sensitive complexometric reagents for the colorimetry of serum zinc that we investigated, one, 2-(5-bromo-2-pyridylazo)-5- diethylaminophenol (Br-PADAP), was found to be a potentially useful compound for trace-metal determinations. It has a high molar absorptivity (120 000 L mol-1 cm-1) but is not convenient to use because it is not very soluble in water. The other reagent, a related pyridylazo compound, is 2-(5-bromo-2-pyridylazo)-5-(N-n-propyl-N-3- sulfopropylamino)phenol (5-BR-PAPS). It seems better suited for use in routine zinc determinations because, besides being water soluble, it has a higher molar absorptivity, 130 000 L mol-1 cm-1. Results by the proposed method developed with 5-Br-PAPS correlated well with those by atomic absorption spectrophotometry. The between-run CV for control sera was less than 5%; the within-run CV (same controls) was less than 4%.




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Copyright © 1985 by the American Association for Clinical Chemistry.