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Clinical Chemistry 31: 1314-1316, 1985;
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Clinical Chemistry, Vol 31, 1314-1316, Copyright © 1985 by American Association for Clinical Chemistry

Binding of Al by protein in plasma of patients on maintenance hemodialysis

M Cochran, D Patterson, S Neoh, B Stevens and R Mazzachi

Gel filtration of plasma from hemodialysis patients, with use of reagents and apparatus with carefully minimized background Al concentrations, reproducibly showed a single peak for Al, corresponding exactly to the elution position of transferrin. The Al/transferrin molar ratio in adjacent fractions was constant (mean 0.126, SE 0.006) in replicate experiments. In contrast, the association of Al with albumin varied. Using both equilibrium dialysis and gel-filtration techniques, in the presence and absence of calcium or phosphate, we could demonstrate no significant binding of Al by human albumin at Al concentrations of 1 to 12 mumol/L. We saw no Al peak in pooled, concentrated, low-molecular-mass fractions of plasma gel-filtered on Sephadex G-50. Evidently, transferrin is the sole Al-binding protein in plasma of hemodialysis patients.




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Copyright © 1985 by the American Association for Clinical Chemistry.