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Clinical Chemistry 31: 1371-1373, 1985;
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Clinical Chemistry, Vol 31, 1371-1373, Copyright © 1985 by American Association for Clinical Chemistry

Liquid-chromatographic measurement of riboflavin in serum and urine with isoriboflavin as internal standard

WE Lambert, PM Cammaert and AP De Leenheer

We describe how riboflavin can be precisely and accurately measured in serum and urine. A structural analog, isoriboflavin, is used as an internal standard. Urine samples are prepared by adding the internal standard and trichloroacetic acid. For serum, proteins are denatured with trichloroacetic acid. A simple Sep-pak treatment of the supernate removes contaminating interferents. Within- and between-run precision is reflected by respective CVs of 2.2 and 4.9% for urine at 180 micrograms/L and 4.4 and 7.3% for serum at 10 micrograms/L. The standard curve is linear far beyond the concentrations encountered in serum and urine. The detection limit is estimated to be 10 micrograms/L and 1 microgram/L for urine and serum, respectively. The normal reference interval, as determined from 50 results for each matrix, is 36 to 349 micrograms/g of creatinine (mean 112 micrograms/g) for urine and 5.5 to 14.4 micrograms/L (mean 8.8 micrograms/L) for serum. Both distributions are skewed. The method is suited for routine use.


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O. Midttun, S. Hustad, E. Solheim, J. Schneede, and P. M. Ueland
Multianalyte Quantification of Vitamin B6 and B2 Species in the Nanomolar Range in Human Plasma by Liquid Chromatography-Tandem Mass Spectrometry
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S. Hustad, P. M. Ueland, and J. Schneede
Quantification of Riboflavin, Flavin Mononucleotide, and Flavin Adenine Dinucleotide in Human Plasma by Capillary Electrophoresis and Laser-induced Fluorescence Detection
Clin. Chem., June 1, 1999; 45(6): 862 - 868.
[Abstract] [Full Text] [PDF]


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