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Clinical Chemistry 31: 1500-1505, 1985;
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Clinical Chemistry, Vol 31, 1500-1505, Copyright © 1985 by American Association for Clinical Chemistry

Simultaneous determination of total IgE and allergen-specific IgE in serum by the MAST chemiluminescent assay system

CR Brown, KW Higgins, K Frazer, LK Schoelz, JW Dyminski, VA Marinkovich, SP Miller and JF Burd

We have developed a chemiluminescent immunoenzymometric system. The first commercial application of this chemiluminescent assay (CLA) is the measurement of total IgE and allergen-specific IgE in human serum. The CLA system is a second-generation adaptation of the MAST RIA allergy profiling system. The MAST CLA system assay protocol consists of three steps: overnight incubation of serum, a 4-h incubation with enzyme-labeled antibody, and a 30-min chemiluminescent reaction, which produces a visible image (immunograph) on high-speed Polaroid instant film. The densities of the bands produced on the film are quantified with an inexpensive microprocessor-controlled infrared transmittance densitometer. The novel luminogenic substrates used yield a constant light output for over 2 h with an intensity at least 10-fold greater than that of commercial chemiluminescent reagents. The MAST CLA system exhibits sensitivity, specificity, and precision equal to that of the MAST RIA system (r = 0.96 for 40 serum samples analyzed with 25 allergens). As many as 35 different allergens per sample can be quantified in a single assay. The MAST CLA system requires no standard curve or volume-dependent pipetting steps, incorporates both positive and negative controls for each sample, and quantifies allergen-specific IgE at picomolar concentrations.


The following articles in journals at HighWire Press have cited this article:


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 Clin. Chem.Home page
N. Honda, U. Lindberg, P. Andersson, S. Hoffmann, and H. Takei
Simultaneous Multiple Immunoassays in a Compact Disc-Shaped Microfluidic Device Based on Centrifugal Force
Clin. Chem., October 1, 2005; 51(10): 1955 - 1961.
[Abstract] [Full Text] [PDF]


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Copyright © 1985 by the American Association for Clinical Chemistry.