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Clinical Chemistry 32: 1832-1835, 1986;
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Clinical Chemistry, Vol 32, 1832-1835, Copyright © 1986 by American Association for Clinical Chemistry

Use of monoclonal and polyclonal antibodies to detect prostatic acid phosphatase by immunoblotting

PC Patel, L Aubin and J Cote

We investigated two techniques of immunoblotting--the Western blot and the dot blot--for use in detecting prostatic acid phosphatase (PAP, EC 3.1.3.2). We used polyclonal antisera to human PAP, produced in rabbits by hyperimmunization with purified PAP, and PAP-specific monoclonal antibodies in the immunoenzymatic protocols. We conclude that PAP can be readily detected by Western blots with use of polyclonal antisera, but not with monoclonal antibodies. On the other hand, using a dot blot assay, we could easily detect PAP with both polyclonal and monoclonal antibodies.




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Copyright © 1986 by the American Association for Clinical Chemistry.