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Clinical Chemistry 32: 1901-1905, 1986;
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Clinical Chemistry, Vol 32, 1901-1905, Copyright © 1986 by American Association for Clinical Chemistry

Production and certification of secondary enzyme reference materials (ERMs). Part 1: Preparation of the sera and some of their properties

JC Koedam, GM Steentjes, S Buitenhuis, E Schmidt and R Klauke

We produced three batches of a human-serum-based enzyme reference material (ERM) enriched with human aspartate aminotransferase (EC 2.6.1.1), alanine aminotransferase (EC 2.6.1.2), creatine kinase (EC 2.7.3.2), and lactate dehydrogenase (EC 1.1.1.27). The added enzymes were not exhaustively purified; thus the final ERMs contained some enzymes as contaminants, of which only glutamate dehydrogenase activity might interfere. The stability during storage and after reconstitution was good. The commutability of the four enzymes in the three ERM batches was also good, except when German or Scandinavian methods for aminotransferases were involved. The temperature-conversion factors for the ERMs were equivalent to those for patients' sera. Reactivation after reconstitution was complete within 5 min and was independent of the temperature of the reconstitution fluid. We believe that these secondary ERMs will aid in the transfer of accuracy between well- defined reference methods and daily working methods so that clinical enzymology results will become more comparable from laboratory to laboratory.




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Copyright © 1986 by the American Association for Clinical Chemistry.