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Clinical Chemistry 32: 2070-2073, 1986;
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Clinical Chemistry, Vol 32, 2070-2073, Copyright © 1986 by American Association for Clinical Chemistry

A simple radial immunodiffusion method for assay of beta 2- microglobulin in serum

JT Wu, F Clayton, S Myers and J Knight

In this method for clinical measurement of beta 2-microglobulin, a 10 g/L agarose gel containing anti-beta 2-microglobulin is used, with subsequent staining with Coomassie Blue. Although the method is slow (requiring 30 h), it is inexpensive, reliable, and accurate over the range of 1 to 10 mg/L, and is useful in the determination of beta 2- microglobulin in serum and cerebrospinal fluid. A modified procedure in which staining with silver is used may be sufficiently sensitive for the clinical assay of urine.





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