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Clinical Chemistry 32: 2083-2084, 1986;
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Clinical Chemistry, Vol 32, 2083-2084, Copyright © 1986 by American Association for Clinical Chemistry

Lentil-lectin-reactive alpha-fetoprotein in the differential diagnosis of benign and malignant liver disease

PK Buamah, R Harris, OF James and AW Skillen

Affinity chromatography of serum on lentil lectin bound to Sepharose 4B has been used to identify different forms of alpha-fetoprotein in serum of patients with liver disease. We studied eight patients with non- malignant liver disease, finding that 7-15% of the serum alpha- fetoprotein bound to the lectin. In contrast, for 15 patients with malignant liver disease, 25-83% of the serum alpha-fetoprotein bound to the lectin. Evidently, this simple technique can be used effectively to differentiate these two conditions.


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Y. Sato, K. Nakata, Y. Kato, M. Shima, N. Ishii, T. Koji, K. Taketa, Y. Endo, and S. Nagataki
Early Recognition of Hepatocellular Carcinoma Based on Altered Profiles of Alpha-Fetoprotein
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Copyright © 1986 by the American Association for Clinical Chemistry.