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Clinical Chemistry 32: 2094-2097, 1986;
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Clinical Chemistry, Vol 32, 2094-2097, Copyright © 1986 by American Association for Clinical Chemistry

6 beta-hydroxycortisol in serum and urine as determined by enzyme immunoassay on microtitre plates

A Zhiri, HA Mayer, V Michaux, M Wellman-Bednawska and G Siest

We modified our previous enzyme immunoassay (Clin Chim Acta 1986;157:267-76) for estimating 6 beta-hydroxycortisol (6 beta-OHF) to provide a more routine procedure for measuring it in urine and to increase sensitivity for its measurement in serum. Precision, analytical recovery, specificity, and detection limit are reported. 6 beta-OHF and 17-hydroxycorticosteroids in 24-h and 4-h (08:00-12:00 h) urine collections from healthy volunteers showed no significant day-to- day differences in excretion rate for either collection period. Mean values for 6 beta-OHF in serum of men, women, and women taking oral contraceptives showed no significant differences among these groups.




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Copyright © 1986 by the American Association for Clinical Chemistry.