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Clinical Chemistry, Vol 32, 283-286, Copyright © 1986 by American Association for Clinical Chemistry
JE Groener, RW Pelton and GM Kostner
This simple, routine assay for measuring cholesteryl ester transfer/exchange activity in human plasma is based on the removal of interfering lipoproteins--very-low-density (VLDL) and low-density lipoproteins (LDL)--by precipitation with polyethylene glycol. High- density lipoproteins (HDL) in the samples do not affect the results. The supernate after precipitation is mixed with [14C]cholesteryl ester- labeled LDL as donor and with HDL as the acceptor for the cholesteryl ester. After incubation for 16 h at 37 degrees C, LDL is separated from HDL by precipitation with dextran sulfate and the radioactivity measured in the supernate, which contains the HDL. The assay is applicable to samples containing as much as 10 mmol of triglycerides per liter. The within-assay CV was 2.7%, the day-to-day CV 6.8%. Results compared well with those by conventional procedures.
The following articles in journals at HighWire Press have cited this article:
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  S. Nakanishi, R. Vikstedt, S. Soderlund, M. Lee-Rueckert, A. Hiukka, C. Ehnholm, M. Muilu, J. Metso, J. Naukkarinen, L. Palotie, et al. Serum, but not monocyte macrophage foam cells derived from low HDL-C subjects, displays reduced cholesterol efflux capacity J. Lipid Res., February 1, 2009; 50(2): 183 - 192. [Abstract] [Full Text] [PDF]
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J.-F. Mauger, A. H Lichtenstein, L. M Ausman, S. M Jalbert, M. Jauhiainen, C. Ehnholm, and B. Lamarche Effect of different forms of dietary hydrogenated fats on LDL particle size Am. J. Clinical Nutrition, September 1, 2003; 78(3): 370 - 375. [Abstract] [Full Text] [PDF]
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A. Soro, M. Jauhiainen, C. Ehnholm, and M.-R. Taskinen Determinants of low HDL levels in familial combined hyperlipidemia J. Lipid Res., August 1, 2003; 44(8): 1536 - 1544. [Abstract] [Full Text] [PDF]
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R. Freese, G. Alfthan, M. Jauhiainen, S. Basu, I. Erlund, I. Salminen, A. Aro, and M. Mutanen High intakes of vegetables, berries, and apples combined with a high intake of linoleic or oleic acid only slightly affect markers of lipid peroxidation and lipoprotein metabolism in healthy subjects Am. J. Clinical Nutrition, November 1, 2002; 76(5): 950 - 960. [Abstract] [Full Text] [PDF]
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J. Vakkilainen, M. Jauhiainen, K. Ylitalo, I. O. Nuotio, J. S. A. Viikari, C. Ehnholm, and M.-R. Taskinen LDL particle size in familial combined hyperlipidemia: effects of serum lipids, lipoprotein-modifying enzymes, and lipid transfer proteins J. Lipid Res., April 1, 2002; 43(4): 598 - 603. [Abstract] [Full Text] [PDF]
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R. Gander, P. Eller, S. Kaser, I. Theurl, D. Walter, T. Sauper, A. Ritsch, J. R. Patsch, and B. Foger Molecular characterization of rabbit phospholipid transfer protein: choroid plexus and ependyma synthesize high levels of phospholipid transfer protein J. Lipid Res., April 1, 2002; 43(4): 636 - 645. [Abstract] [Full Text] [PDF]
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A. H. Lichtenstein, L. M. Ausman, S. M. Jalbert, M. Vilella-Bach, M. Jauhiainen, S. McGladdery, A. T. Erkkila, C. Ehnholm, J. Frohlich, and E. J. Schaefer Efficacy of a Therapeutic Lifestyle Change/Step 2 diet in moderately hypercholesterolemic middle-aged and elderly female and male subjects J. Lipid Res., February 1, 2002; 43(2): 264 - 273. [Abstract] [Full Text] [PDF]
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N. R. Matthan, K. Cianflone, A. H. Lichtenstein, L. M. Ausman, M. Jauhiainen, and P. J. H. Jones Hydrogenated fat consumption affects acylation- stimulating protein levels and cholesterol esterification rates in moderately hypercholesterolemic women J. Lipid Res., November 1, 2001; 42(11): 1841 - 1848. [Abstract] [Full Text] [PDF]
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A. H. Lichtenstein, M. Jauhiainen, S. McGladdery, L. M. Ausman, S. M. Jalbert, M. Vilella-Bach, C. Ehnholm, J. Frohlich, and E. J. Schaefer Impact of hydrogenated fat on high density lipoprotein subfractions and metabolism J. Lipid Res., April 1, 2001; 42(4): 597 - 604. [Abstract] [Full Text]
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 H. Helisten, A. Höckerstedt, K. Wähälä, A. Tiitinen, H. Adlercreutz, M. Jauhiainen, and M. J. Tikkanen Accumulation of High-Density Lipoprotein-Derived Estradiol-17{beta} Fatty Acid Esters in Low-Density Lipoprotein Particles J. Clin. Endocrinol. Metab., March 1, 2001; 86(3): 1294 - 1300. [Abstract] [Full Text]
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J. A. M. Beentjes, A. van Tol, W. J. Sluiter, and R. P. F. Dullaart Effect of growth hormone replacement therapy on plasma lecithin:cholesterol acyltransferase and lipid transfer protein activities in growth hormone-deficient adults J. Lipid Res., June 1, 2000; 41(6): 925 - 932. [Abstract] [Full Text]
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 M. C. Jong, W. L. Hendriks, L. C. van Vark, V. E. H. Dahlmans, J. E. M. Groener, and L. M. Havekes Oxidized VLDL Induces Less Triglyceride Accumulation in J774 Macrophages Than Native VLDL Due to an Impaired Extracellular Lipolysis Arterioscler Thromb Vasc Biol, January 1, 2000; 20(1): 144 - 151. [Abstract] [Full Text] [PDF]
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S. C. Riemens, A. Van Tol, W. J. Sluiter, and R. P. F. Dullaart Acute and chronic effects of a 24-hour intravenous triglyceride emulsion challenge on plasma lecithin: cholesterol acyltransferase, phospholipid transfer protein, and cholesteryl ester transfer protein activities J. Lipid Res., August 1, 1999; 40(8): 1459 - 1466. [Abstract] [Full Text]
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S. C. Riemens, A. Van Tol, B. K. Stulp, and R. P. F. Dullaart Influence of insulin sensitivity and the TaqIB cholesteryl ester transfer protein gene polymorphism on plasma lecithin:cholesterol acyltransferase and lipid transfer protein activities and their response to hyperinsulinemia in non-diabetic men J. Lipid Res., August 1, 1999; 40(8): 1467 - 1474. [Abstract] [Full Text]
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M. Jauhiainen, J. Huuskonen, M. Baumann, J. Metso, T. Oka, T. Egashira, H. Hattori, V. M. Olkkonen, and C. Ehnholm Phospholipid transfer protein (PLTP) causes proteolytic cleavage of apolipoprotein A-I J. Lipid Res., April 1, 1999; 40(4): 654 - 664. [Abstract] [Full Text]
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R. M. Fisher, H. Burke, V. Nicaud, C. Ehnholm, and S. E. Humphries Effect of variation in the apo A-IV gene on body mass index and fasting and postprandial lipids in the European Atherosclerosis Research Study II J. Lipid Res., February 1, 1999; 40(2): 287 - 294. [Abstract] [Full Text]
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 D. V.-v. Bruggen, I. Kalkman, T. van Gent, A. van Tol, and H. Jansen Induction of Adrenal Scavenger Receptor BI and Increased High Density Lipoprotein-Cholesteryl Ether Uptake by in Vivo Inhibition of Hepatic Lipase J. Biol. Chem., November 27, 1998; 273(48): 32038 - 32041. [Abstract] [Full Text] [PDF]
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K.-A. Rye, M. Jauhiainen, P. J. Barter, and C. Ehnholm Triglyceride-enrichment of high density lipoproteins enhances their remodelling by phospholipid transfer protein J. Lipid Res., March 1, 1998; 39(3): 613 - 622. [Abstract] [Full Text]
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P. J. Pussinen, M. Jauhiainen, J. Metso, L. E. Pyle, Y. L. Marcel, N. H. Fidge, and C. Ehnholm Binding of phospholipid transfer protein (PLTP) to apolipoproteins A-I and A-II: location of a PLTP binding domain in the amino terminal region of apoA-I J. Lipid Res., January 1, 1998; 39(1): 152 - 161. [Abstract] [Full Text]
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A. Ritsch, H. Drexel, F. W. Amann, C. Pfeifhofer, and J. R. Patsch Deficiency of Cholesteryl Ester Transfer Protein : Description of the Molecular Defect and the Dissociation of Cholesteryl Ester and Triglyceride Transport in Plasma Arterioscler Thromb Vasc Biol, December 1, 1997; 17(12): 3433 - 3441. [Abstract] [Full Text]
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M. J. Liinamaa, M. L. Hannuksela, Y. A. Kesaniemi, and M. J. Savolainen Altered Transfer of Cholesteryl Esters and Phospholipids in Plasma From Alcohol Abusers Arterioscler Thromb Vasc Biol, November 1, 1997; 17(11): 2940 - 2947. [Abstract] [Full Text]
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H. E. Miettinen, M. Jauhiainen, H. Gylling, S. Ehnholm, A. Palomaki, T. A. Miettinen, and K. Kontula Apolipoprotein A-IFIN (Leu159->Arg) Mutation Affects Lecithin : Cholesterol Acyltransferase Activation and Subclass Distribution of HDL but Not Cholesterol Efflux From Fibroblasts Arterioscler Thromb Vasc Biol, November 1, 1997; 17(11): 3021 - 3032. [Abstract] [Full Text]
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B. Foger, A. Ritsch, A. Doblinger, H. Wessels, and J. R. Patsch Relationship of Plasma Cholesteryl Ester Transfer Protein to HDL Cholesterol: Studies in Normotriglyceridemia and Moderate Hypertriglyceridemia Arterioscler Thromb Vasc Biol, December 1, 1996; 16(12): 1430 - 1436. [Abstract] [Full Text]
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M. L. Hannuksela, M. Rantala, Y. A. Kesaniemi, and M. J. Savolainen Ethanol-Induced Redistribution of Cholesteryl Ester Transfer Protein (CETP) Between Lipoproteins Arterioscler Thromb Vasc Biol, February 1, 1996; 16(2): 213 - 221. [Abstract] [Full Text]
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