Clinical Chemistry Link to Randox Laboratories Web Site
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 32: 351-353, 1986;
This Article
Right arrow Full Text (PDF)
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Delahunty, T.
Right arrow Articles by Hollander, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Delahunty, T.
Right arrow Articles by Hollander, D.

Clinical Chemistry, Vol 32, 351-353, Copyright © 1986 by American Association for Clinical Chemistry

New liquid-chromatographic method for measuring polyethylene glycol in urine

T Delahunty and D Hollander

The various Mr fractions of polyethylene glycol (PEG) in human urine are quantified by "high-pressure" liquid chromatography. A simple preparation step involving lyophilization and chloroform extraction of the sample is required. Aliquots (10 microL) are chromatographed isocratically in equivolume mixtures of methanol and water on a column of styrene divinylbenzene and the refractive index of the effluent is measured. The results vary linearly with the concentrations of standards up to at least 10 g/L, and the six major fractions are clearly identifiable in injected samples containing 2 g or more of total PEG per liter. As little as 4 g/L can be precisely quantified, but for assessing the individual fractions, we recommend a minimum sample concentration of 7 g/L. Analytical recovery of PEG added to urine controls was 90%. Urines collected during 6 h from 11 human subjects after each had ingested 5.6 g of PEG showed no interference from endogenous compounds. We find this method to be a simple, labor- saving means of quantifying urinary PEG in the clinical laboratory.




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1986 by the American Association for Clinical Chemistry.