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Clinical Chemistry, Vol 32, 358-360, Copyright © 1986 by American Association for Clinical Chemistry
RR Little, JD England, HM Wiedmeyer, EM McKenzie, R Mitra, PM Erhart, JB Durham and DE Goldstein
As the clinical utility of glycated hemoglobin (gHb) measurement increases, so does the need for standardization of values between different methods and different laboratories. Using three different methods, we examined the feasibility of interlaboratory standardization of gHb measurement. A liquid-chromatographic (HPLC) system from our research laboratory was designated the reference method. For gHb standards we used erythrocyte hemolysates prepared from blood samples from nondiabetic and diabetic subjects. Values assigned to each standard were based on the mean of multiple gHb determinations by the HPLC method. A clinical laboratory routinely prepared hemolysates and assayed gHb by commercially available ion-exchange ("mini column") and affinity chromatographic methods. For each assay a standard curve was constructed and gHb values were derived from these curves. Samples analyzed in the clinical laboratory were also analyzed in the research laboratory and the curve-derived values were compared with the HPLC- measured values, to determine the accuracy of our interlaboratory standardization procedure. Correlations were excellent (r = 0.99). The lack of significant differences between calculated and HPLC-measured values indicates that interlaboratory standardization is feasible.
The following articles in journals at HighWire Press have cited this article:
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G. Halwachs-Baumann, S. Katzensteiner, W. Schnedl, P. Purstner, T. Pieber, and M. Wilders-Truschnig Comparative evaluation of three assay systems for automated determination of hemoglobin A1c Clin. Chem., March 1, 1997; 43(3): 511 - 517. [Abstract] [Full Text] [PDF]
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S. Kataoka, D. C. Robbins, L. D. Cowan, O. Go, J. L. Yeh, R. B. Devereux, R. R. Fabsitz, E. T. Lee, T. K. Welty, and B. V. Howard Apolipoprotein E Polymorphism in American Indians and Its Relation to Plasma Lipoproteins and Diabetes: The Strong Heart Study Arterioscler Thromb Vasc Biol, August 1, 1996; 16(8): 918 - 925. [Abstract] [Full Text]
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