Clinical Chemistry AACC Online Job Center
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 32: 368-370, 1986;
This Article
Right arrow Full Text (PDF)
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Johnson, R. N.
Right arrow Articles by Baker, J. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Johnson, R. N.
Right arrow Articles by Baker, J. R.

Clinical Chemistry, Vol 32, 368-370, Copyright © 1986 by American Association for Clinical Chemistry

The alkaline reducing activity of glycated serum proteins and its relevance to diabetes mellitus

RN Johnson and JR Baker

We investigated the ability of the fructosamine assay to detect glycation of serum proteins. We incubated both whole human serum and serum protein fractions in vitro with [14C]glucose, and analyzed for reducing activity and for uptake of 14C by protein. In all experiments, the reducing activity increased linearly with time for seven days and was correlated with 14C uptake (r = 0.94-0.98). Protein ketoamines were about fivefold more actively reducing than equimolar concentrations of deoxymorpholinofructose, the fructosamine standard, which explains why values for fructosamine in serum are higher than the expected concentration of protein ketoamines. We also used [14C, 2-3H]glucose to assess the contribution of the aldimine component to 14C uptake. Whole human serum and albumin incubated with [14C, 2-3H]glucose showed little uptake of 3H in relation to 14C. We conclude that glycated protein can be simply and reliably quantified by the fructosamine assay, and we discuss the relevance of this conclusion to the monitoring of diabetes.




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1986 by the American Association for Clinical Chemistry.