| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Clinical Chemistry, Vol 32, 476-481, Copyright © 1986 by American Association for Clinical Chemistry
RJ Thompson, AP Jackson and N Langlois
With a two-site CK-MB assay, we screened serum samples from 1008 blood donors for the presence of antibodies to mouse monoclonal immunoglobulin. These antibodies were capable of cross-linking the labeled antibody with the solid-phase antibody in the two-site assay, thus generating a falsely high apparent CK-MB concentration. In 92 (9.12%) of the blood donors tested, apparent CK-MB concentrations of 10- 1000 micrograms/L decreased to less than 3 micrograms/L when re-assayed with non-immune mouse serum (10 mL/L) included in the assay reagent. We tested the ability of non-immune sera from other animal species to lower the concentration of apparent CK-MB in 58 of the 92 samples. Bovine and ovine serum were almost as effective as mouse serum; feline, canine, and rabbit serum were less effective. Of the samples tested, 12% (1.1% of the original population screened) showed apparent CK-MB values that either were not depressed by bovine serum or were only partly depressed. We discuss the possible etiology of these antibodies in normal subjects and recommend that all mouse monoclonal two-site assays should contain non-immune mouse serum (or a suitable "irrelevant" mouse monoclonal antibody) to prevent false-positive results.
The following articles in journals at HighWire Press have cited this article:
|
|
 |
|
  D. J. Warren, J. Bjerner, E. Paus, O. P. Bormer, and K. Nustad Use of an In Vivo Biotinylated Single-Chain Antibody as Capture Reagent in an Immunometric Assay to Decrease the Incidence of Interference from Heterophilic Antibodies Clin. Chem., May 1, 2005; 51(5): 830 - 838. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Eriksson, H. Halenius, K. Pulkki, J. Hellman, and K. Pettersson Negative Interference in Cardiac Troponin I Immunoassays by Circulating Troponin Autoantibodies Clin. Chem., May 1, 2005; 51(5): 839 - 847. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Roongsritong, I. Warraich, and C. Bradley Common Causes of Troponin Elevations in the Absence of Acute Myocardial Infarction: Incidence and Clinical Significance Chest, May 1, 2004; 125(5): 1877 - 1884. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. B. Martins, B. M. Pasi, C. M. Litwin, and H. R. Hill Heterophile Antibody Interference in a Multiplexed Fluorescent Microsphere Immunoassay for Quantitation of Cytokines in Human Serum Clin. Vaccine Immunol., March 1, 2004; 11(2): 325 - 329. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Kessimian Laboratory Medicine and Diagnostic Testing J Am Podiatr Med Assoc, March 1, 2004; 94(2): 194 - 197. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Eriksson, M. Junikka, P. Laitinen, K. Majamaa-Voltti, H. Alfthan, and K. Pettersson Negative Interference in Cardiac Troponin I Immunoassays from a Frequently Occurring Serum and Plasma Component Clin. Chem., July 1, 2003; 49(7): 1095 - 1104. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. L. Bertholf, L. Johannsen, and G. Benrubi False Elevation of Serum CA-125 Level Caused by Human Anti-Mouse Antibodies Ann. Clin. Lab. Sci., October 1, 2002; 32(4): 414 - 418. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Parhami-Seren, R. Haberly, M. N. Margolies, and G. T. Haupert Jr Ouabain-Binding Protein(s) From Human Plasma Hypertension, August 1, 2002; 40(2): 220 - 228. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. J. Kim, O. F. Laterza, K. G. Hock, J. F. Pierson-Perry, D. M. Kaminski, M. Mesguich, F. Braconnier, R. Zimmermann, M. Zaninotto, M. Plebani, et al. Performance of a Revised Cardiac Troponin Method That Minimizes Interferences from Heterophilic Antibodies Clin. Chem., July 1, 2002; 48(7): 1028 - 1034. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Bjerner, K. Nustad, L. F. Norum, K. H. Olsen, and O. P. Bormer Immunometric Assay Interference: Incidence and Prevention Clin. Chem., April 1, 2002; 48(4): 613 - 621. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Covinsky, O. Laterza, J. D. Pfeifer, T. Farkas-Szallasi, and M. G. Scott An IgM {lambda} Antibody to Escherichia coli Produces False-Positive Results in Multiple Immunometric Assays Clin. Chem., August 1, 2000; 46(8): 1157 - 1161. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. W. Butch Falsely Increased Values for Rabbit Immunoglobulin-based Nephelometric Immunoassays Attributable to Human Anti-Rabbit Antibodies Clin. Chem., August 1, 2000; 46(8): 1207 - 1208. [Full Text] [PDF]
|
|
|
|
|
|
L. J. Kricka Human Anti-Animal Antibody Interferences in Immunological Assays Clin. Chem., July 1, 1999; 45(7): 942 - 956. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Despres and A. M. Grant Antibody interference in thyroid assays: a potential for clinical misinformation Clin. Chem., March 1, 1998; 44(3): 440 - 454. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
