Clinical Chemistry
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Clinical Chemistry 32: 529-532, 1986;
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Clinical Chemistry, Vol 32, 529-532, Copyright © 1986 by American Association for Clinical Chemistry

Different diuresis-dependent excretions of urinary enzymes: N-acetyl- beta-D-glucosaminidase, alanine aminopeptidase, alkaline phosphatase, and gamma-glutamyltransferase

K Jung, G Schulze and C Reinholdt

We studied how much of the lysosomal enzyme N-acetyl-beta-D- glucosaminidase (EC 3.2.1.30) and of the brush-border enzymes alanine aminopeptidase (EC 3.4.11.2), alkaline phosphatase (EC 3.1.3.1), and gamma-glutamyltransferase (EC 2.3.2.2) was excreted in urine over 8 h after a high intake of fluid (22 mL per kilogram of body weight). The hourly excretion of all four enzymes increased with the increasing urine flow rate. The excretion rate of the brush-border enzymes was more markedly influenced than that of N-acetyl-beta-D-glucosaminidase. By relating the enzyme excretion to urinary creatinine we could reduce the variability of brush-border enzyme output and could completely compensate for the effect of diuresis on the excretion of N-acetyl-beta- D-glucosaminidase.


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Copyright © 1986 by the American Association for Clinical Chemistry.