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Clinical Chemistry 32: 545-547, 1986;
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Clinical Chemistry, Vol 32, 545-547, Copyright © 1986 by American Association for Clinical Chemistry

An enzymatic method for calibration of serum lipase assays

GE Hoffmann, U Neumann, S Hoffmann, P Kaspar and L Weiss

An enzymatic method for the calibration of a turbidimetric lipase assay is described, based on measurement of free fatty acids liberated by the action of lipase. The substrate of the turbidimetric assay is a colipase-containing triolein emulsion. For determination of the free fatty acids a commercial test kit including acyl-CoA synthetase, acyl- CoA oxidase, and peroxidase is used. Intra- and interassay imprecision (CV) is about 5% at above-normal lipase activities, about 10% at normal values. Temperature coefficients are 1.24 and 1.45, respectively, for measurements at 30 and 37 degrees C vs 25 degrees C.




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Copyright © 1986 by the American Association for Clinical Chemistry.