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Clinical Chemistry 32: 598-602, 1986;
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Clinical Chemistry, Vol 32, 598-602, Copyright © 1986 by American Association for Clinical Chemistry

Evaluation of absorption and first- and second-derivative spectra for simultaneous quantification of bilirubin and hemoglobin

MF Merrick and HL Pardue

We discuss the relative merits of absorption and first- and second- derivative spectra for the simultaneous quantification of bilirubin and hemoglobin, and evaluate single-, two-, and multiwavelength methods. Although both species can be quantified from single- or two-wavelength absorption data, lipids or other absorbing or light-scattering components introduce systematic errors that can be substantially decreased by using first- or second-derivative spectra. Multi- wavelength data-processing methods with derivative spectra permit quantification of components with overlapping spectra and decrease the random error usually associated with derivative methods. A typical least-squares equation for quantifying bilirubin in the presence of hemoglobin and bovine serum albumin from multi-wavelength second- derivative data is y(computed) = 0.999x(prepared) + 0.00 mg/L.


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V K Hughes, P S Ellis, T Burt, and N E I Langlois
The practical application of reflectance spectrophotometry for the demonstration of haemoglobin and its degradation in bruises
J. Clin. Pathol., April 1, 2004; 57(4): 355 - 359.
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Copyright © 1986 by the American Association for Clinical Chemistry.