Clinical Chemistry Link to Randox Laboratories Web Site
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 32: 637-640, 1986;
This Article
Right arrow Full Text (PDF)
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Toivonen, E.
Right arrow Articles by Lovgren, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Toivonen, E.
Right arrow Articles by Lovgren, T.

Clinical Chemistry, Vol 32, 637-640, Copyright © 1986 by American Association for Clinical Chemistry

Two-site time-resolved immunofluorometric assay of human insulin

E Toivonen, I Hemmila, J Marniemi, PN Jorgensen, J Zeuthen and T Lovgren

We describe a two-site "sandwich"-type time-resolved immunofluorometric assay for human insulin, based on use of two monoclonal antibodies with different specificities. The first antibody is immobilized on the surface of microtiter plate strip wells, the other is labeled with Eu3+. Serum samples can be assayed with one incubation step; two incubation steps are required when plasma samples are assayed. After the immunoreactions are complete, the bound fraction of Eu3+-label is quantified by dissociating it in a fluorescence-enhancement solution and measuring its fluorescence with a fluorometer with time-resolution. The sensitivity of the assay is 0.24 micro-int. units/mL. The standard curve is linear from 0.24 to 2400 micro-int. units/mL.


The following articles in journals at HighWire Press have cited this article:


Home page
 NEJMHome page
P. Hovi, S. Andersson, J. G. Eriksson, A.-L. Jarvenpaa, S. Strang-Karlsson, O. Makitie, and E. Kajantie
Glucose Regulation in Young Adults with Very Low Birth Weight
N. Engl. J. Med., May 17, 2007; 356(20): 2053 - 2063.
[Abstract] [Full Text] [PDF]

Home page
 J Biomol ScreenHome page
F. Poulsen and K. B. Jensen
A Luminescent Oxygen Channeling Immunoassay for the Determination of Insulin in Human Plasma
J Biomol Screen, March 1, 2007; 12(2): 240 - 247.
[Abstract] [PDF]

Home page
 CirculationHome page
A. Handberg, K. Levin, K. Hojlund, and H. Beck-Nielsen
Identification of the Oxidized Low-Density Lipoprotein Scavenger Receptor CD36 in Plasma: A Novel Marker of Insulin Resistance
Circulation, September 12, 2006; 114(11): 1169 - 1176.
[Abstract] [Full Text] [PDF]

Home page
 J ANIM SCIHome page
N. B. Kristensen and D. L. Harmon
Effects of adding valerate, caproate, and heptanoate to ruminal buffers on splanchnic metabolism in steers under washed-rumen conditions
J Anim Sci, August 1, 2005; 83(8): 1899 - 1907.
[Abstract] [Full Text] [PDF]

Home page
 J ANIM SCIHome page
N. B. Kristensen and D. L. Harmon
Effect of increasing ruminal butyrate absorption on splanchnic metabolism of volatile fatty acids absorbed from the washed reticulorumen of steers
J Anim Sci, December 1, 2004; 82(12): 3549 - 3559.
[Abstract] [Full Text] [PDF]

Home page
 Diabetes CareHome page
K. Levin, O. Hother-Nielsen, J. E. Henriksen, and H. Beck-Nielsen
Effects of Troglitazone in Young First-Degree Relatives of Patients With Type 2 Diabetes
Diabetes Care, January 1, 2004; 27(1): 148 - 154.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
H. B.T. Christesen, B. B. Jacobsen, S. Odili, C. Buettger, A. Cuesta-Munoz, T. Hansen, K. Brusgaard, O. Massa, M. A. Magnuson, C. Shiota, et al.
The Second Activating Glucokinase Mutation (A456V): Implications for Glucose Homeostasis and Diabetes Therapy
Diabetes, April 1, 2002; 51(4): 1240 - 1246.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
K. Hojlund, M. Wildner-Christensen, O. Eshoj, C. Skjarbak, J. J. Holst, O. Koldkjar, D. Moller Jensen, and H. Beck-Nielsen
Reference intervals for glucose, {beta}-cell polypeptides, and counterregulatory factors during prolonged fasting
Am J Physiol Endocrinol Metab, January 1, 2001; 280(1): E50 - E58.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1986 by the American Association for Clinical Chemistry.