Clinical Chemistry
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 32: 693-696, 1986;
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lindgren, K.
Right arrow Articles by Rodopoulos, N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lindgren, K.
Right arrow Articles by Rodopoulos, N.

Clinical Chemistry, Vol 32, 693-696, Copyright © 1986 by American Association for Clinical Chemistry

Determination of vanillylmandelic acid with ion-pair chromatography and fluorescence detection

K Lindgren and N Rodopoulos

We describe a chromatographic procedure for sensitive (2.0 mumol/L), specific quantification of vanillylmandelic acid (VMA) in urine with iso-VMA as an internal standard. After rapid extraction from urine, the VMA is determined by isocratic reversed-phase ion-pair chromatography on a bonded C8 column and detection of the native fluorescence on excitation at 285 nm. The fluorescence signal is quite dependent on the pH of the mobile phase. Results by the method vary linearly with VMA concentration up to 320 mumol/L and correlate well (r = 0.9880) with those obtained by conventional ultraviolet spectrophotometry. The mean 24-h excretion of VMA from 29 healthy volunteers was 21.4 (SD 5.4) mumol.




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1986 by the American Association for Clinical Chemistry.