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Clinical Chemistry 32: 835-839, 1986;
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Clinical Chemistry, Vol 32, 835-839, Copyright © 1986 by American Association for Clinical Chemistry

Direct determination of sulfate conjugates of 17-oxosteroids in urine by liquid chromatography

O Nishikaze and J Iwata

We describe a new liquid-chromatographic method with fluorescence detection for direct simultaneous determination of four urinary sulfate conjugates of 17-oxosteroids (17OS), without use of solvolysis or enzymatic hydrolysis. The 17OS sulfates in urine samples are extracted with benzene as ion pairs in the presence of benzyltributylammonium chloride. These ion pairs are converted to hydrazones by use of dansyl hydrazine in trichloroacetic acid solution and then are separated by liquid chromatography. The proposed method, being simple and rapid, shows a good separation of 17OS sulfates and superior sensitivity, sufficient recovery, and good reproducibility.




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Copyright © 1986 by the American Association for Clinical Chemistry.