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Clinical Chemistry 32: 854-856, 1986;
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Clinical Chemistry, Vol 32, 854-856, Copyright © 1986 by American Association for Clinical Chemistry

Radioimmunoassay of prolactin in blood spotted on filter paper

F Bassett, BA Gross and CJ Eastman

In this method for estimating prolactin, 50 microL of whole blood obtained by finger puncture is spotted onto filter paper and blood-spot samples are "punched out" with a 3-mm (diameter) paper punch. The blood is extracted with aqueous buffers and the prolactin measured in large batches by radioimmunoassay. Results were identical with those for prolactin in serum. Prolactin in blood spots is stable at room temperature for up to one week and for several months at -20 degrees C. This simple technique for obtaining blood samples for prolactin estimation has particular potential for field studies of lactating women.


The following articles in journals at HighWire Press have cited this article:


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 Am. J. Clin. Nutr.Home page
S. D O'Broin and E. W Gunter
Screening of folate status with use of dried blood spots on filter paper
Am. J. Clinical Nutrition, September 1, 1999; 70(3): 359 - 367.
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Copyright © 1986 by the American Association for Clinical Chemistry.