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Clinical Chemistry 32: 1002-1004, 1986;
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Clinical Chemistry, Vol 32, 1002-1004, Copyright © 1986 by American Association for Clinical Chemistry

Liquid-chromatographic determination of 4-hydroxyproline in urine

H Hughes, L Hagen and RA Sutton

In this method for 4-hydroxyproline in urine, hydroxyproline is derivatized with 4-chloro-7-nitrobenzofurazan, with subsequent estimation by reversed-phase "high-performance" liquid chromatography. The ranges for excretion of free and total hydroxyproline while the subjects were ingesting unrestricted diets were 2-29 and 122-374 mumol/24 h (n = 21), respectively, with no significant sex-related difference. A comparison with results by colorimetry indicated no significant differences: mean (n = 18) concentrations (mumol/L) of hydroxyproline in urine were 180 (SD 149) by the present method, 163 (SD 166) by colorimetry. For protein hydrolysate the respective values were 5.9 (SD 2.7) and 6.7 (SD 2.9).




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Copyright © 1986 by the American Association for Clinical Chemistry.