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Clinical Chemistry, Vol 32, 921-929, Copyright © 1986 by American Association for Clinical Chemistry
GR Cooper, SJ Smith, IW Duncan, A Mather, WD Fellows, T Foley, ID Frantz Jr, JB Gill, TA Grooms and I Hynie
In 1975 the Centers for Disease Control, in cooperation with the American Association for Clinical Chemistry Cholesterol Reference Method Study Group, began an investigation to develop a reference method for total cholesterol. Five potential reference methods were compared with the definitive method developed by the National Bureau of Standards before the chemical method of Abell et al. (J Biol Chem 1952;195:357-66) was selected as the recommended reference method. Because acceptance of a proposed reference method depends so greatly on the method's capability for transfer to other laboratories by written specifications and instructions, a transferability testing study was designed and conducted with 14 laboratories. The study consisted of preliminary testing of readiness of equipment, reagents, and personnel followed by transferability testing with eight runs on 10 serum pools. Laboratoires that did not meet readiness specifications had higher CVs in the transferability testing. The study demonstrated that the proposed method permits laboratories to attain a CV of less than 1.5% for one laboratory and of less than 3.0% among laboratories. The mean percent bias value was less than 1.0% for six of the 14 laboratories, less than 1.5% for 12, and less than 3.0% for all 14 laboratories.
The following articles in journals at HighWire Press have cited this article:
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  R. Cramb, J. French, M. Mackness, R D. G Neely, M. Caslake, and F. MacKenzie Lipid external quality assessment: commutability between external quality assessment and clinical specimens Ann Clin Biochem, May 1, 2008; 45(3): 260 - 265. [Abstract] [Full Text] [PDF]
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 E. T. Bairaktari, K. I. Seferiadis, and M. S. Elisaf Evaluation of Methods for the Measurement of Low-Density Lipoprotein Cholesterol Journal of Cardiovascular Pharmacology and Therapeutics, January 1, 2005; 10(1): 45 - 54. [Abstract] [PDF]
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 M. Nauck, G. R. Warnick, and N. Rifai Methods for Measurement of LDL-Cholesterol: A Critical Assessment of Direct Measurement by Homogeneous Assays versus Calculation Clin. Chem., February 1, 2002; 48(2): 236 - 254. [Abstract] [Full Text] [PDF]
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G. R. Warnick, M. Nauck, and N. Rifai Evolution of Methods for Measurement of HDL-Cholesterol: From Ultracentrifugation to Homogeneous Assays Clin. Chem., September 1, 2001; 47(9): 1579 - 1596. [Abstract] [Full Text] [PDF]
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G. L. Myers, M. M. Kimberly, P. P. Waymack, S. J. Smith, G. R. Cooper, and E. J. Sampson A Reference Method Laboratory Network for Cholesterol: A Model for Standardization and Improvement of Clinical Laboratory Measurements Clin. Chem., November 1, 2000; 46(11): 1762 - 1772. [Abstract] [Full Text] [PDF]
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A. M. Gotto and G. R. Cooper Citation Classics in Lipid Measurement and Applications Clin. Chem., November 1, 1998; 44(11): 2234 - 2237. [Full Text] [PDF]
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 B. M. McManus, A. B. Toth, J. A. Engel, G. L. Myers, H. K. Naito, J. E. Wilson, and G. R. Cooper Progress in Lipid Reporting Practices and Reliability of Blood Cholesterol Measurement in Clinical Laboratories in Nebraska: Efforts to Align Results With the Centers for Disease Control, and Feasibility of Meeting National Cholesterol Education Program Guidelines JAMA, July 7, 1989; 262(1): 83 - 88. [Abstract] [PDF]
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D. D. Koch, D. J. Hassemer, D. A. Wiebe, and R. H. Laessig Testing Cholesterol Accuracy: Performance of Several Common Laboratory Instruments JAMA, November 4, 1988; 260(17): 2552 - 2557. [Abstract] [PDF]
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