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Clinical Chemistry, Vol 32, 967-971, Copyright © 1986 by American Association for Clinical Chemistry
EA Stein, L DiPersio, AJ Pesce, M Kashyap, JT Kao, L Srivastava and C McNerney
We produced a monoclonal antibody (C2-22) to human apolipoprotein (Apo) AII and describe its use in an enzyme-linked immunoabsorbant assay (ELISA) for Apo AII in human plasma and lipoprotein subfractions. No cross reactivity of the antibody with Apo CI, CII, CIII, E, or ablumin was detected. Apo AI and low- and very-low-density lipoprotein cross reacted by 0.25%, less than 0.2%, and less than 0.3%, respectively. Whole plasma high-density lipoprotein (HDL) and HDL subfractions (HDL2 and HDL3) produced parallel displacement curves. This quantitative ELISA is based on competition between solid-phase-bound Apo AII and free Apo AII. Bound C2-22 is detected by alkaline-phosphatase-labeled second antibody. The standard curve for the assay is linear for plasma diluted 500-fold originally containing 140 to 1140 mg of Apo AII per liter. Delipidation of plasma samples exposed no additional antigenic sites. Within- and between-run CVs were respectively 8.4% and 8.7% at 327 mg/L of Apo AII, and 6.8% and 7.4% at 587 mg/L. Results correlated well with those by a polyvalent-antisera-based RIA procedure: r = 0.916, p less than 0.01, RIA = 0.896 ELISA -19.1 mg/L.
The following articles in journals at HighWire Press have cited this article:
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  E. A. Stein, D. R. Illingworth, P. O. Kwiterovich Jr, C. A. Liacouras, M. A. Siimes, M. S. Jacobson, T. G. Brewster, P. Hopkins, M. Davidson, K. Graham, et al. Efficacy and Safety of Lovastatin in Adolescent Males With Heterozygous Familial Hypercholesterolemia: A Randomized Controlled Trial JAMA, January 13, 1999; 281(2): 137 - 144. [Abstract] [Full Text] [PDF]
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 D. L. Rainwater, B. D. Mitchell, M. C. Mahaney, and S. M. Haffner Genetic Relationship Between Measures of HDL Phenotypes and Insulin Concentrations Arterioscler Thromb Vasc Biol, December 1, 1997; 17(12): 3414 - 3419. [Abstract] [Full Text]
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A. G. Comuzzie, D. L. Rainwater, J. Blangero, M. C. Mahaney, J. L. VandeBerg, and J. W. MacCluer Shared and Unique Genetic Effects Among Seven HDL Phenotypes Arterioscler Thromb Vasc Biol, May 1, 1997; 17(5): 859 - 864. [Abstract] [Full Text]
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 D. L. Sprecher, B. V. Harris, E. A. Stein, P. S. Bellet, L. M. Keilson, and L. A. Simbartl Higher Triglycerides, Lower High-Density Lipoprotein Cholesterol, and Higher Systolic Blood Pressure in Lipoprotein Lipase–Deficient Heterozygotes: A Preliminary Report Circulation, December 15, 1996; 94(12): 3239 - 3245. [Abstract] [Full Text]
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B. D. Mitchell, C. M. Kammerer, J. Blangero, M. C. Mahaney, D. L. Rainwater, B. Dyke, J. E. Hixson, R. D. Henkel, R. M. Sharp, A. G. Comuzzie, et al. Genetic and Environmental Contributions to Cardiovascular Risk Factors in Mexican Americans: The San Antonio Family Heart Study Circulation, November 1, 1996; 94(9): 2159 - 2170. [Abstract] [Full Text]
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 E. A. Stein, M. H. Davidson, C. A. Dujovne, D. B. Hunninghake, R. B. Goldberg, D. R. Illingworth, R. H. Knopp, V. T. Miller, P. Frost, J. L. Isaacsohn, et al. Efficacy and Tolerability of Low-dose Simvastatin and Niacin, Alone and in Combination, in Patients With Combined Hyperlipidemia: A Prospective Trial Journal of Cardiovascular Pharmacology and Therapeutics, April 1, 1996; 1(2): 107 - 116. [Abstract] [PDF]
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A. G. Comuzzie, J. Blangero, M. C. Mahaney, R. M. Sharp, J. L. VandeBerg, M. P. Stern, and J. W. MacCluer Triiodothyronine Exerts a Major Pleiotropic Effect on Reverse Cholesterol Transport Phenotypes Arterioscler Thromb Vasc Biol, February 1, 1996; 16(2): 289 - 293. [Abstract] [Full Text]
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A. T.K. Singh, D. L. Rainwater, S. M. Haffner, J. L. VandeBerg, W. R. Shelledy, P. H. Moore Jr, and T. D. Dyer Effect of Diabetes on Lipoprotein Size Arterioscler Thromb Vasc Biol, November 1, 1995; 15(11): 1805 - 1811. [Abstract] [Full Text]
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D. L. Rainwater, M. J. Ludwig, S. M. Haffner, and J. L. VandeBerg Lipid and Lipoprotein Factors Associated With Variation in Lp(a) Density Arterioscler Thromb Vasc Biol, March 1, 1995; 15(3): 313 - 319. [Abstract] [Full Text]
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 W. Insull Jr, D. Black, C. Dujovne, J. D. Hosking, D. Hunninghake, L. Keilson, R. Knopp, J. McKenney, E. Stein, A. J. Troendle, et al. Efficacy and Safety of Once-Daily vs Twice-Daily Dosing With Fluvastatin, a Synthetic Reductase Inhibitor, in Primary Hypercholesterolemia Arch Intern Med, November 14, 1994; 154(21): 2449 - 2455. [Abstract] [PDF]
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D. R. Illingworth, E. A. Stein, Y. B. Mitchel, C. A. Dujovne, P. H. Frost, R. H. Knopp, P. Tun, R. V. Zupkis, and R. A. Greguski Comparative Effects of Lovastatin and Niacin in Primary Hypercholesterolemia: A Prospective Trial Arch Intern Med, July 25, 1994; 154(14): 1586 - 1595. [Abstract] [PDF]
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 D. B. Hunninghake, E. A. Stein, C. A. Dujovne, W. S. Harris, E. B. Feldman, V. T. Miller, J. A. Tobert, P. M. Laskarzewski, E. Quiter, J. Held, et al. The Efficacy of Intensive Dietary Therapy Alone or Combined with Lovastatin in Outpatients with Hypercholesterolemia N. Engl. J. Med., April 29, 1993; 328(17): 1213 - 1219. [Abstract] [Full Text]
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 M.C. Houston, L. Olafsson, and M.C. Burger Effects of Nifedipine GITS and Atenolol Monotherapy on Serum Lipids, Blood Pressure, Heart Rate, and Weight in Mild to Moderate Hypertension Angiology, September 1, 1991; 42(9): 681 - 690. [Abstract] [PDF]
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R. R. Wing, K. A. Matthews, L. H. Kuller, E. N. Meilahn, and P. L. Plantinga Weight Gain at the Time of Menopause Arch Intern Med, January 1, 1991; 151(1): 97 - 102. [Abstract] [PDF]
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E. Stein, R. Kreisberg, V. Miller, G. Mantell, L. Washington, and D. R. Shapiro Effects of Simvastatin and Cholestyramine in Familial and Nonfamilial Hypercholesterolemia Arch Intern Med, February 1, 1990; 150(2): 341 - 345. [Abstract] [PDF]
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C. Deck and K. Radack Effects of Modest Doses of Omega-3 Fatty Acids on Lipids and Lipoproteins in Hypertriglyceridemic Subjects: A Randomized Controlled Trial Arch Intern Med, August 1, 1989; 149(8): 1857 - 1862. [Abstract] [PDF]
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The Lovastatin Study Group III A Multicenter Comparison of Lovastatin and Cholestyramine Therapy for Severe Primary Hypercholesterolemia JAMA, July 15, 1988; 260(3): 359 - 366. [Abstract] [PDF]
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 R. J. HAVEL, D. B. HUNNINGHAKE, D. R. ILLINGWORTH, R. S. LEES, E. A. STEIN, J. A. TOBERT, S. R. BACON, J. A. BOLOGNESE, P. H. FROST, G. E. LAMKIN, et al. Lovastatin (Mevinolin) in the Treatment of Heterozygous Familial Hypercholesterolemia: A Multicenter Study Ann Intern Med, November 1, 1987; 107(5): 609 - 615. [Abstract] [PDF]
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Therapeutic Response to Lovastatin (Mevinolin) in Nonfamilial Hypercholesterolemia: A Multicenter Study JAMA, November 28, 1986; 256(20): 2829 - 2834. [Abstract] [PDF]
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