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Clinical Chemistry 32: 987-991, 1986;
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Clinical Chemistry, Vol 32, 987-991, Copyright © 1986 by American Association for Clinical Chemistry

A rapid enzymatic procedure for "fingerprinting" bacteria by using pattern recognition of two-dimensional fluorescence data

CP Pau, G Patonay, CW Moss, GM Carlone, TM Rossi and IM Warner

We describe a rapid fluorescence technique for bacterial "fingerprinting," based on the differences in enzyme content and activity of various bacteria. Bacterial cells are incubated with a mixture of carefully chosen fluorogenic enzyme substrates to produce fluorophores with unique emission and excitation properties. The resulting two-dimensional fluorescence spectrum of the product mixture produces a pattern characteristic of the bacterium. A Fourier-transform- based pattern recognition algorithm is used for spectral matching and for differentiating visually similar spectra. This sensitive technique is potentially applicable to differentiation and identification of bacteria in clinical laboratories.


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Microbiol. Mol. Biol. Rev., March 1, 2003; 67(1): 38 - 51.
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Copyright © 1986 by the American Association for Clinical Chemistry.