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Clinical Chemistry 32: 1303-1306, 1986;
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Clinical Chemistry, Vol 32, 1303-1306, Copyright © 1986 by American Association for Clinical Chemistry

Immunoassays for low concentrations of albumin in urine

A Silver, A Dawnay, J Landon and WR Cattell

We have developed and validated simple, rapid immunoassays to measure concentrations of albumin in urine ranging from 5 to 200 mg/L. We use antiserum to human albumin, raised in sheep, and we separate the antibody-bound and free fractions of albumin by using a second antiserum, produced in donkeys, against the Fc fragment of sheep IgG. These two antisera can be mixed and added to assay tubes as a single reagent without inhibiting antigen binding. Samples or standards are incubated for at least 30 min with fluorescein-labeled albumin and the premixed antiserum reagent. After brief centrifugation, the supernate is discarded and the precipitate containing the bound fraction is dissolved and its fluorescence measured. Alternatively, 125I-labeled albumin can be used as tracer with all other reagents unchanged; in this case one simply counts the radioactivity of the bound fraction. A reference interval of less than 0.2-2.8 mg/mmol of creatinine was obtained for untimed, daytime, midstream urine specimens from 80 healthy subjects.


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 Arch Intern MedHome page
J. Thompson, M. Reid, I. Hambleton, and G. R. Serjeant
Albuminuria and Renal Function in Homozygous Sickle Cell Disease: Observations From a Cohort Study
Arch Intern Med, April 9, 2007; 167(7): 701 - 708.
[Abstract] [Full Text] [PDF]


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Copyright © 1986 by the American Association for Clinical Chemistry.