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Clinical Chemistry 32: 1323-1327, 1986;
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Clinical Chemistry, Vol 32, 1323-1327, Copyright © 1986 by American Association for Clinical Chemistry

Laser-excited immunofluorometric assay of prolactin, with use of antibodies coupled to lanthanide-labeled diethylenetriaminepentaacetic acid

H Dechaud, R Bador, F Claustrat and C Desuzinges

We describe an immunofluorometric assay for prolactin based on lanthanide labeling of a monoclonal antibody and measuring time- resolved fluorescence. In this "sandwich"-type assay, the label (Eu3+) was bound to the second antibody by means of a simple, rapid method involving the anhydride of diethylenetriaminepentaacetic acid. To measure the photoluminescence of europium (or other lanthanides), we have developed a time-resolved fluorometer with a nitrogen laser as the pulsed excitation source. During the assay, the solid-phase antibody immobilized inside a polystyrene tube is incubated with the plasma sample and the second antibody in a one-step procedure. Results for 67 human plasmas correlated well (r = 0.98) with those by an immunoradiometric method.




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Copyright © 1986 by the American Association for Clinical Chemistry.