Clinical Chemistry Link to Randox Laboratories Web Site
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 32: 1383-1386, 1986;
This Article
Right arrow Full Text (PDF)
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Offenbacher, E. G.
Right arrow Articles by Pi-Sunyer, F. X.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Offenbacher, E. G.
Right arrow Articles by Pi-Sunyer, F. X.

Clinical Chemistry, Vol 32, 1383-1386, Copyright © 1986 by American Association for Clinical Chemistry

Rapid enzymatic pretreatment of samples before determining chromium in serum or plasma

EG Offenbacher, HJ Dowling, CJ Rinko and FX Pi-Sunyer

A simplified method for preparing blood serum and plasma for Zeeman electrothermal atomic absorption spectrometry is described and applied to the measurement of chromium in human serum and plasma. This enzymatic degradation with bacterial protease (EC 3.4.21.3) requires little laboratory apparatus, decreases the work and time of sample preparation, and obviates some potential sources of contamination. We used bovine reference serum (USDA No. 7292) to validate Cr concentration. There was less Cr in serum than in plasma, whether sodium heparin or sodium citrate was used as anticoagulant. For six human subjects, Cr in serum averaged 0.15 (SD 0.02) micrograms/L, 0.26 (SD 0.03) micrograms/L in heparinized plasma, and 0.28 (SD 0.02) micrograms/L in citrated plasma. We postulate that the Cr concentration is lower in serum because some of the Cr binds to proteins complexed with the clot in the coagulation process.




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1986 by the American Association for Clinical Chemistry.