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Clinical Chemistry 32: 1389-1393, 1986;
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Clinical Chemistry, Vol 32, 1389-1393, Copyright © 1986 by American Association for Clinical Chemistry

Use of the caffeine reagent in direct spectrophotometry of bilirubin

KL Vink, W Schuurman and R van Gansewinkel

The molar absorptivity of unconjugated bilirubin in caffeine reagent is independent of the protein matrix. This finding, together with the simplicity of a dilution step for direct spectrophotometry, will improve the calibration methods of bilirubin and make them more nearly accurate. This is encouraging for the development of a new method for bilirubin determination in neonates; moreover, the caffeine reagent has a clearing influence on the turbidity of human sera. These findings should also be important for standardization, especially because the method of Jendrassik and Grof is also protein-independent. Therefore, the introduction of one reliable, inexpensive, "universal" standard of bilirubin in bovine serum albumin will be of importance for both methods.


The following articles in journals at HighWire Press have cited this article:


Home page
 Clin. Chem.Home page
S. F. Lo, B. T. Doumas, and E. R. Ashwood
Performance of Bilirubin Determinations in US Laboratories--Revisited
Clin. Chem., January 1, 2004; 50(1): 190 - 194.
[Abstract] [Full Text] [PDF]


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