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Clinical Chemistry 32: 1394-1397, 1986;
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Clinical Chemistry, Vol 32, 1394-1397, Copyright © 1986 by American Association for Clinical Chemistry

Use of a quality-control plasma sample to decrease interassay variation in radioimmunoassays of apolipoprotein A-I

YP Au, BJ Hallaway and BA Kottke

The apolipoprotein A-I (apo A-I) radioimmunoassay established in our laboratory involves use of purified apo A-I as the primary standard for quantifying apo A-I in plasma and a pooled plasma (quality-control sample) as a secondary standard to decrease interassay variation. The measured values for apo A-I increased over time as the labeled antigen degraded. We observed these results in two separate studies: (a) apo A- I in plasma from 16 subjects was measured 12 times during four months, and (b) apo A-I in a single pooled plasma was measured 48 times during 10 months. We show that use of a quality-control plasma sample as a secondary standard decreased interassay variation, which was, in part, ascribable to degradation of the labeled antigen.




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Copyright © 1986 by the American Association for Clinical Chemistry.