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Clinical Chemistry 32: 1407-1409, 1986;
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Clinical Chemistry, Vol 32, 1407-1409, Copyright © 1986 by American Association for Clinical Chemistry

Liquid-chromatographic analysis for cyclosporine with use of a microbore column and small sample volume

T Annesley, K Matz, L Balogh, L Clayton and D Giacherio

This liquid-chromatographic assay requires 0.2 to 0.5 mL of whole blood, avoids the use of diethyl ether, and consumes only 10 to 20% of the solvents used in prior methods. Sample preparation involves an acidic extraction with methyl-t-butyl ether, performed in a 13 X 100 mm disposable glass tube, then a short second extraction of the organic phase with sodium hydroxide. After evaporation of the methyl-t-butyl ether, chromatography is performed on an "Astec" 2.0-mm (i.d.) octyl column. We compared results by this procedure with those by use of earlier larger-scale extractions and their respective 4.6-mm (i.d.) columns; analytical recoveries of cyclosporins A and D were comparable with previous findings and results for patients' specimens were equivalent, but the microbore columns provided greatly increased resolution and sensitivity.




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Copyright © 1986 by the American Association for Clinical Chemistry.