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Clinical Chemistry 32: 1464-1467, 1986;
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Clinical Chemistry, Vol 32, 1464-1467, Copyright © 1986 by American Association for Clinical Chemistry

Fluorometry of nanogram amounts of selenium in biological samples

Y Tamari, S Ohmori and K Hiraki

For fluorometry of selenium in human blood, hair, and liver and in leaves, we wet-ashed the samples with conventional nitric and perchloric acids, and then extracted piazselenol (complex of Se and 2,3- diaminonaphthalene) in cyclohexane. Selenium was back-extracted from the cyclohexane into nitric acid to remove the fluorometric interferences of trace amounts of organic compounds. This fluorometric method is rapid and suitable for routine analysis. We applied the method to human hair samples and compared it with the data for non- destructive neutron activation analysis of the hair.




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Copyright © 1986 by the American Association for Clinical Chemistry.