Clinical Chemistry
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 32: 1542-1544, 1986;
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Delahunty, T.
Right arrow Articles by Hollander, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Delahunty, T.
Right arrow Articles by Hollander, D.

Clinical Chemistry, Vol 32, 1542-1544, Copyright © 1986 by American Association for Clinical Chemistry

Liquid-chromatographic method for estimating urinary sugars: applicability to studies of intestinal permeability

T Delahunty and D Hollander

Sugars of exogenous origin excreted in the urine can be rapidly quantified by "high-pressure" liquid chromatography. A simple extraction with an ion-exchange resin is used to prepare the sample for analysis. Aliquots (20 microL) are chromatographed on a cation-exchange column at 85 degrees C, with water as the mobile phase. Sugars are detected with a refractive index detector. Lactulose, rhamnose, and mannitol all give discrete peaks and a linear response up to 5 g/L, with analytical recoveries from urine of 80, 62, and 80%, respectively. Precision is good, the CVs for lactulose, rhamnose, and mannitol being 2.9, 4.0, and 5.6%, respectively. The only endogenous compound consistently present in the chromatograms is urea, which does not interfere. However, glucosuria, if present, could interfere with the lactulose estimation. This method may be a simple, labor-saving means of quantifying urinary sugars in the clinical laboratory.


The following articles in journals at HighWire Press have cited this article:


Home page
 J. Am. Coll. Nutr.Home page
M. Navarro and R. J. Wood
Plasma Changes in Micronutrients Following a Multivitamin and Mineral Supplement in Healthy Adults
J. Am. Coll. Nutr., April 1, 2003; 22(2): 124 - 132.
[Abstract] [Full Text] [PDF]

Home page
 JPEN J Parenter Enteral NutrHome page
A. L. Buchman, A. A. Moukarzel, S. Bhuta, M. Belle, M. E. Ament, C. D. Eckhert, D. Hollander, J. Gornbeln, J. D. Kopple, and S. R. Vijayaroghavan
Parenteral Nutrition Is Associated With Intestinal Morphologic and Functional Changes in Humans
JPEN J Parenter Enteral Nutr, November 1, 1995; 19(6): 453 - 460.
[Abstract] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1986 by the American Association for Clinical Chemistry.