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Clinical Chemistry 32: 1779-1781, 1986;
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Clinical Chemistry, Vol 32, 1779-1781, Copyright © 1986 by American Association for Clinical Chemistry

Ultramicromethod for measuring the activity of terminal deoxynucleotidyl transferase

B Leotard, M Marchand-Arvier and C Vigneron

We describe an ultramicromethod for measurement of terminal deoxynucleotidyl transferase activity. The materials used are as proposed by Hosli (Clin Chem 1977;23:1476-84) for antenatal diagnosis. The technique is based on the methods of Beutler and Kuhl (Am J Clin Pathol 1978;70:733-7) and Yasmineh et al. (Clin Chem 1980; 26:891-5). The procedure makes it possible to reduce the blood sample to 2 mL at the most and to reduce the cost of the measurement very considerably. The technique gives results similar to those given by the conventional techniques.




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Copyright © 1986 by the American Association for Clinical Chemistry.