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Clinical Chemistry 33: 132-134, 1987;
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Clinical Chemistry, Vol 33, 132-134, Copyright © 1987 by American Association for Clinical Chemistry

Quantification of desferrioxamine in blood plasma by inductively coupled plasma atomic emission spectrometry

S Bourdon, P Houze and R Bourdon

A sensitive method, inductively coupled plasma atomic emission spectroscopy, is used to measure desferrioxamine in blood plasma. The desferrioxamine is transformed into its iron chelate, ferrioxamine, which is extracted into benzyl alcohol, then re-extracted into HCl (0.5 mol/L), which is used as the sample for the spectroscopy. For a 0.5-mL plasma sample, the detection limit (1 microgram/mL) suffices for following the concentration of desferrioxamine in plasma after its subcutaneous or intramuscular injection (40 mg per kg of body weight). Neither blood pigments nor trace metals interfere.


The following articles in journals at HighWire Press have cited this article:


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 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
Y.-C. T. Huang, A. J. Ghio, E. Nozik-Grayck, and C. A. Piantadosi
Vascular release of nonheme iron in perfused rabbit lungs
Am J Physiol Lung Cell Mol Physiol, March 1, 2001; 280(3): L474 - L481.
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Copyright © 1987 by the American Association for Clinical Chemistry.