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Clinical Chemistry 33: 135-137, 1987;
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Clinical Chemistry, Vol 33, 135-137, Copyright © 1987 by American Association for Clinical Chemistry

Variables affecting resolution of lung phospholipids in one-dimensional thin-layer chromatography

J Krahn

Resolution of the confusion in the literature about the separation of lung phospholipids in thin-layer chromatographic systems has awaited a systematic study of the variables that potentially affect this separation. In this study I show that: incorporation of ammonium sulfate into silica gel "GHL" has a dramatic effect on separation of lung phospholipids; this effect is equally dramatic but different in activated and nonactivated gels; when it picks up moisture, ammonium sulfate-activated gel very rapidly loses its ability to resolve lecithin from phosphatidylinositol; in gel containing ammonium sulfate, small amounts of phosphatidylethanolamine are hydrolyzed to lyso- phosphatidylethanolamine.




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Copyright © 1987 by the American Association for Clinical Chemistry.