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Clinical Chemistry 33: 48-51, 1987;
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Clinical Chemistry, Vol 33, 48-51, Copyright © 1987 by American Association for Clinical Chemistry

Europium and samarium as labels in time-resolved immunofluorometric assay of follitropin

R Bador, H Dechaud, F Claustrat and C Desuzinges

This time-resolved immunofluorometric assay for human follitropin involves use of europium- or samarium-labeled monoclonal antibodies, with an average incorporation ratio of 3 mol of Eu3+ or Sm3+ per mole of antibody. These lanthanide ions are bound to the antibody molecules by means of the anhydride of diethylenetriaminepentaacetic acid. The solid-phase antibody is immobilized inside polystyrene tubes in which plasma samples were assayed in a one-step procedure. After incubation, the fluorescence intensity of Eu3+ or Sm3+ label is measured by time- resolved fluorometry, with a nitrogen laser as the pulsed excitation source. The sensitivity of the assay is largely better with Eu3+ than with Sm3+ because of the difference in their intrinsic luminescence properties. Results obtained with the proposed methods correlated well with those by an immunoradiometric method.


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Copyright © 1987 by the American Association for Clinical Chemistry.