Clinical Chemistry
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Clinical Chemistry 33: 1731-1735, 1987;
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Clinical Chemistry, Vol 33, 1731-1735, Copyright © 1987 by American Association for Clinical Chemistry

Measurement of acetylcholinesterase in erythrocytes in the field

RA Magnotti Jr, JP Eberly, DE Quarm and RS McConnell
Department of Internal Medicine, University of Cincinnati Medical Center, OH 45267-0585.

We describe here a field method we developed for colorimetry of erythrocytic acetylcholinesterase (EC 3.1.1.7) in capillary blood samples. Three stable, premixed assay reagents and de-ionized water (but no centrifuge or balance) are required. This method, adapted for a microplate format, is essentially that of Ellman et al. (Biochem Pharmacol 1961;7:88-95) as modified by George and Abernethy (Clin Chem 1983;29:365-8). Assays were quantified and corrected for hematocrit by using a battery-powered colorimeter with a silicon carbide (blue) light- emitting-diode source. Advantages over existing field methods include better portability, ruggedness, greater precision, and lower cost per sample.




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Copyright © 1987 by the American Association for Clinical Chemistry.