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Clinical Chemistry 33: 1832-1836, 1987;
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Clinical Chemistry, Vol 33, 1832-1836, Copyright © 1987 by American Association for Clinical Chemistry

Antibodies to porcine uteroferrin used in measurement of human tartrate- resistant acid phosphatase

ZO Echetebu, TM Cox and DW Moss
Department of Chemical Pathology, Royal Postgraduate Medical School, Hammersmith Hospital, London, U.K.

The immunological similarity between human tartrate-resistant acid phosphatase (EC 3.1.3.2) and porcine uteroferrin previously reported for the isoenzyme from spleens of patients with leukemic reticuloendotheliosis (Ketcham et al., J Biol Chem 1985;260:5768-76) has been confirmed for partly purified acid phosphatase found in the spleen of a patient with Gaucher's disease, and for the corresponding isoenzyme in other tissues and serum. Anti-uteroferrin antibodies raised in rabbits have been used to demonstrate the feasibility of their application in an immunoassay for tartrate-resistant acid phosphatase in serum.


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