Clinical Chemistry
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Clinical Chemistry 33: 1882-1884, 1987;
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Clinical Chemistry, Vol 33, 1882-1884, Copyright © 1987 by American Association for Clinical Chemistry

Immunonephelometry of apolipoprotein B with a centrifugal analyzer

NQ Hanson, TS LeGeault and EF Freier
Department of Laboratory Medicine and Pathology, University of Minnesota Medical School, Minneapolis.

We developed an automated immunonephelometric assay for the measurement of apolipoprotein B (apo B) with a light-scattering microcentrifugal analyzer. Pretreating specimens with a dilute solution of Tween 20 or triglyceride lipase decreased the nephelometric response of apo B. Polyethylene glycol is included in the reaction mixture, and the reaction is complete within 4 min. The method is precise (CV = 6.5%, mean = 0.68 g/L) and the standard curve is linear to an apo B concentration of 2.8 g/L. Lipemia does not interfere with the method if grossly lipemic specimens are centrifuged to remove chylomicrons.


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T. J. Wilt, R. P. Lofgren, K. L. Nichol, A. E. Schorer, L. Crespin, D. Downes, and J. Eckfeldt
Fish Oil Supplementation Does Not Lower Plasma Cholesterol in Men with Hypercholesterolemia: Results of a Randomized, Placebo-Controlled Crossover Study
Ann Intern Med, December 1, 1989; 111(11): 900 - 905.
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Copyright © 1987 by the American Association for Clinical Chemistry.