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Clinical Chemistry 33: 1886-1887, 1987;
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Clinical Chemistry, Vol 33, 1886-1887, Copyright © 1987 by American Association for Clinical Chemistry

Simplified sodium dodecyl sulfate-polyacrylamide gel electrophoresis of unconcentrated urine with enhanced resolution and detection sensitivity

T Marshall and KM Williams
Biology Department, University of Ulster, Coleraine, Northern Ireland.

We applied a simple sodium dodecyl sulfate-polyacrylamide gel electrophoresis method to urine. The method, developed for serum protein analysis (Clin Chem 1984;30:475-9), has a high sample throughput and gives excellent resolution with unconcentrated urine. It clearly distinguishes and characterizes proteinuric urine (7.5 microL) by Coomassie Blue staining and gives complex silver-stained patterns with nonproteinuric urine (2 microL). The former is recommended for routine clinical screening, the latter for research purposes.


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Copyright © 1987 by the American Association for Clinical Chemistry.