Clinical Chemistry
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Clinical Chemistry 33: 1898-1900, 1987;
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Clinical Chemistry, Vol 33, 1898-1900, Copyright © 1987 by American Association for Clinical Chemistry

Liquid chromatography and fluorescence polarization immunoassay methods compared for measuring flecainide acetate in serum

RJ Straka, TJ Hoon, RL Lalonde, JA Pieper and MB Bottorff
Department of Clinical Pharmacy, College of Pharmacy, University of Tennessee, Memphis 38163.

We analyzed 99 patients' serum samples for concentrations of a new antiarrhythmic agent, flecainide acetate, by fluorescence polarization immunoassay (FPIA) and "high-performance" liquid chromatography (HPLC). Within-day and between-day coefficients of variation at concentrations in the low and high ends of the therapeutic range were less than 7% for HPLC and less than 9% for FPIA. There was no statistical difference in the mean (+/- SD) concentrations of the clinical serum samples measured by the two methods (607 +/- 334 micrograms/L by HPLC, 602 +/- 344 micrograms/L by FPIA), but results by each differed by a mean of 0.13%. FPIA and HPLC measurements correlated significantly (r = 0.98, P less than 0.05), and were linearly related (slope = 0.970, intercept = 13 micrograms/L) as assessed by orthogonal regression. Both assay methods produced similar concentration measurements and were sufficiently accurate and precise to be used in therapeutic drug monitoring.




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Copyright © 1987 by the American Association for Clinical Chemistry.