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Clinical Chemistry 33: 1903-1906, 1987;
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Clinical Chemistry, Vol 33, 1903-1906, Copyright © 1987 by American Association for Clinical Chemistry

Single-reagent polarization fluoroimmunoassay of methamphetamine in urine

SA Eremin, G Gallacher, H Lotey, DS Smith and J Landon
Department of Chemistry, Moscow State University, U.S.S.R.

We developed a polarization fluoroimmunoassay for the specific detection of methamphetamine in urine. Sheep antiserum raised against methamphetamine was premixed with a fluorescein-labeled amphetamine tracer in a single reagent. The use of labeled amphetamine gave a more sensitive and more specific assay than did the use of a labeled methamphetamine tracer. The assay system had equal sensitivity for the d- and l-isomers of methamphetamine while the cross reactivity of amphetamine and other structurally related compounds was 3% or less. With a positive/negative cutoff concentration set at 1 mg/L, the polarization fluoroimmunoassay reliably identified human urines that had been reported positive for methamphetamine by an established gas- liquid chromatographic method.


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P. H. Lee and D. J. Bevis
Development of a Homogeneous High Throughput Fluorescence Polarization Assay for G Protein-Coupled Receptor Binding
J Biomol Screen, December 1, 2000; 5(6): 415 - 419.
[Abstract] [PDF]


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Copyright © 1987 by the American Association for Clinical Chemistry.