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Clinical Chemistry 33: 1921-1923, 1987;
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Clinical Chemistry, Vol 33, 1921-1923, Copyright © 1987 by American Association for Clinical Chemistry

Multiple drugs of abuse in urine detected with a single reagent and fluorescence polarization

DL Colbert and M Childerstone
Department of Chemical Pathology, St. Bartholomew's Hospital, London, U.K.

We describe a simple polarization fluoroimmunoassay for the detection of several commonly abused drugs in urine. The single reagent used, prepared by mixing four different antisera and three fluorescein- labeled derivatives, produces a polarization signal that is the average of the individual signals of the derivatives. One adds urine to the pre- mixed reagent, incubates at room temperature for a few minutes, then measures fluorescence polarization. The presence of any of several abused drugs, at concentrations of 1 mg/L or more, noticeably decreases the average signal. Although other combinations are possible, the present assay detects the presence of a cocaine metabolite, amphetamine, and (or) several barbiturates.




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Copyright © 1987 by the American Association for Clinical Chemistry.