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Clinical Chemistry 33: 2019-2023, 1987;
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Clinical Chemistry, Vol 33, 2019-2023, Copyright © 1987 by American Association for Clinical Chemistry

Specific immunoradiometric assay of insulin-like growth factor I with use of monoclonal antibodies

MG Scott, GC Cuca, JR Petersen, LR Lyle, BD Burleigh and WH Daughaday
Mallinckrodt, Inc., Diagnostic Products Division, St. Louis, MO 63042.

We identified two monoclonal antibodies that bind spatially distinct epitopes on insulin-like growth factor I (IGF-I). Using these two antibodies, we developed a simultaneous, two-site immunoradiometric assay (IRMA) specific for IGF-I. This IRMA has no detectable cross reactivity with insulin, proinsulin, prolactin, or somatotropin, and less than 2% crossreactivity with IGF-II. The assay response varies linearly with IGF-I concentrations of 0-800 micrograms/L in serum; the detection limit is about 10 micrograms/L. A comparison of 26 IGF-I serum values from the IRMA and from a previously reported IGF-I specific RIA gave a correlation coefficient of 0.96 with no substantial bias (slope = 1.10). IGF-I values for serum, as an aid in assessing growth abnormalities, are easily (only three pipetting steps) obtained in less than 4 h.


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