Clinical Chemistry
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Clinical Chemistry 33: 2100-2106, 1987;
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Clinical Chemistry, Vol 33, 2100-2106, Copyright © 1987 by American Association for Clinical Chemistry

Improved measurement of urinary total protein (including light-chain proteins) with a Coomassie brilliant blue G-250-sodium dodecyl sulfate reagent

DK Wimsatt and JA Lott
Department of Pathology, Ohio State University, Columbus 43210.

The Coomassie Brilliant Blue G-250 method for urinary proteins underestimates urinary immunoglobulin light chains when albumin or pooled serum is used as the protein standard. The specific color yields of these and other proteins can be brought closer together by adding sodium dodecyl sulfate to the reagent; however, there is some loss of sensitivity. We found such a reagent to be satisfactory for assaying urinary proteins on studying 43 patients with light-chain proteinuria, 19 of whom had multiple myeloma and six possible multiple myeloma.


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T. Marshall and K. M. Williams
Total Protein Determination in Urine: Elimination of a Differential Response between the Coomassie Blue and Pyrogallol Red Protein Dye-binding Assays
Clin. Chem., March 1, 2000; 46(3): 392 - 398.
[Abstract] [Full Text] [PDF]


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Copyright © 1987 by the American Association for Clinical Chemistry.